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產品名稱：重組人類單核細胞趨化蛋白-3 / CCL7蛋白
產品型號：rHuMCP-3/CCL7
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類單核細胞趨化蛋白-3 / CCL7蛋白與其它公司提供的重組蛋白不同，rHuMCP-3/CCL7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類單核細胞趨化蛋白-3 / CCL7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類單核細胞趨化蛋白-3 / CCL7蛋白

Synonyms NC28, Small-inducible cytokine A7

Species Human

Accession P80098

GeneID 6354

Source Escherichia coli.

Molecular Weight 重組人類單核細胞趨化蛋白-3 / CCL7蛋白Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance 重組人類單核細胞趨化蛋白-3 / CCL7蛋白Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人類單核細胞趨化蛋白-3 / CCL7蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.

重組人類單核細胞趨化蛋白-3 / CCL7蛋白

SDS-PAGE

Reference 1. Opdenakker G, Fiten P, Nys G, et al. 1994. Genomics. 21:403-8.
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.

Background Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.

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Synonyms	NC28, Small-inducible cytokine A7
Species	Human
Accession	P80098
GeneID	6354
Source	Escherichia coli.
Molecular Weight	重組人類單核細胞趨化蛋白-3 / CCL7蛋白Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	重組人類單核細胞趨化蛋白-3 / CCL7蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人類單核細胞趨化蛋白-3 / CCL7蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
重組人類單核細胞趨化蛋白-3 / CCL7蛋白
SDS-PAGE
Reference	1. Opdenakker G, Fiten P, Nys G, et al. 1994. Genomics. 21:403-8. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42.
Background	Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.

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產品名稱：重組人類單核細胞趨化蛋白-3 / CCL7蛋白