產品詳情
簡單介紹：
重組人白介素-31蛋白與其它公司提供的重組蛋白不同，rHuIL-31蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白介素-31蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白介素-31蛋白
| Species | Human |
| Accession | Q6EBC2 |
| GeneID | 386653 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | 重組人白介素-31蛋白SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組人白介素-31蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-31 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人白介素-31蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白介素-31蛋白 | |
| Reference |
1. Sonkoly E, Muller A, Lauerma AI, et al. 2006. J Allergy Clin Immunol, 117: 411-7. 2. Jawa RS, Chattopadhyay S, Tracy E, et al. 2008. J Interferon Cytokine Res, 28: 207-19. 3. Takaoka A, Arai I, Sugimoto M, et al. 2005. Eur J Pharmacol, 516: 180-1. 4. Ohmatsu H, Sugaya M, Suga H, et al. 2012. Acta Derm Venereol, 92: 282-3 |
| Background | Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31. |
