產品詳情
簡單介紹：
重組人類DES1-3胰島素樣生長因子1蛋白與其它公司提供的重組蛋白不同，rHuDES1-3 IGF-1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類DES1-3胰島素樣生長因子1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類DES1-3胰島素樣生長因子1蛋白
| Synonyms | DES1-3 IGF-I, DES(1-3) IGF-I, IGF-I DES(1-3), DES1-3/Insulin-Like Growth factor 1 |
| Species | Human |
| Accession | P05019 |
| GeneID | 3479 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類DES1-3胰島素樣生長因子1蛋白Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 67 amino acids. |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Physical Appearance | 重組人類DES1-3胰島素樣生長因子1蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 50 EU/mg of rHuDES1-3 IGF-1 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人類DES1-3胰島素樣生長因子1蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類DES1-3胰島素樣生長因子1蛋白 | |
| SDS-PAGE |
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| Reference |
1. Ross M, Francis GL, Szabo L, et al. 1989. Biochem J, 258: 267-72. 2. Kummer A, Pulford BE, Ishii DN, et al. 2003. Int J Exp Diabesity Res, 4: 45-57. |
| Background | IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. DES(1-3)IGF-1, is a truncated variant of human IGF-1 with the tripeptide Gly-Pro-Glu absent from the N-terminus. It has been isolated from bovine colostrum, human brain and porcine uterus. The DES(1-3)IGF-1 probably results from post-translational cleavage of IGF-1. It has about 10-fold more potent than IGF-1 at stimulating hypertrophy and proliferation of cultured cells, a consequence of much reduced binding to IGF-binding proteins, in turn caused by the absence of the glutamate at position 3. Clinical opportunities for DES(1-3)IGF-1 have not yet been evaluated, but could apply in catabolic states as well as for the treatment of inflammatory bowel diseases. |
