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產品名稱：重組恒河猴血清淀粉樣蛋白A1蛋白
產品型號：rRhSAA1
產品廠商：KALANG
產品文檔：

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重組恒河猴血清淀粉樣蛋白A1蛋白與其它公司提供的重組蛋白不同，rRhSAA1蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組恒河猴血清淀粉樣蛋白A1蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組恒河猴血清淀粉樣蛋白A1蛋白

Synonyms Amyloid fibril protein AA

Species Rhesus Macaque

Accession F6V9N7

GeneID 694827

Source Escherichia coli.

Molecular Weight 重組恒河猴血清淀粉樣蛋白A1蛋白Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組恒河猴血清淀粉樣蛋白A1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRhSAA1 as determined by LAL method.

Reconstitution 重組恒河猴血清淀粉樣蛋白A1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組恒河猴血清淀粉樣蛋白A1蛋白

SDS-PAGE

Reference 1. Stevens G, Ramsay M, Kluve-Beckerman B, et al. 1993. Genomics, 15: 242-3.
2. Kluve-Beckerman BandSong M. 1995. Gene, 159: 289-90.
3. Kluve-Beckerman B, Malle E, Vitt H, et al. 1991. Biochem Biophys Res Commun, 181: 1097-102.
4. Milan E, Lazzari C, Anand S, et al. 2012. J Proteomics, 76 Spec No.: 91-101.

Background Serum Amyloid A protein-1 belongs to the SAA family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). It is a multifunctional protein produced by hepatocytes in response to pro-inflammatory cytokines. SAA1 is a ligand for CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE on monocytes/macrophages, inducing chemotaxis and generation of cytokines and tissue factor. It also can bind the surface of invading gram-negative bacteria, acting as an opsonin to aid clearance by macrophages. Additionally, SAA1 binds platelets, probably by engaging fibrinogen on the platelet surface.

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Synonyms	Amyloid fibril protein AA
Species	Rhesus Macaque
Accession	F6V9N7
GeneID	694827
Source	Escherichia coli.
Molecular Weight	重組恒河猴血清淀粉樣蛋白A1蛋白Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組恒河猴血清淀粉樣蛋白A1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRhSAA1 as determined by LAL method.
Reconstitution	重組恒河猴血清淀粉樣蛋白A1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組恒河猴血清淀粉樣蛋白A1蛋白
SDS-PAGE
Reference	1. Stevens G, Ramsay M, Kluve-Beckerman B, et al. 1993. Genomics, 15: 242-3. 2. Kluve-Beckerman BandSong M. 1995. Gene, 159: 289-90. 3. Kluve-Beckerman B, Malle E, Vitt H, et al. 1991. Biochem Biophys Res Commun, 181: 1097-102. 4. Milan E, Lazzari C, Anand S, et al. 2012. J Proteomics, 76 Spec No.: 91-101.
Background	Serum Amyloid A protein-1 belongs to the SAA family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). It is a multifunctional protein produced by hepatocytes in response to pro-inflammatory cytokines. SAA1 is a ligand for CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE on monocytes/macrophages, inducing chemotaxis and generation of cytokines and tissue factor. It also can bind the surface of invading gram-negative bacteria, acting as an opsonin to aid clearance by macrophages. Additionally, SAA1 binds platelets, probably by engaging fibrinogen on the platelet surface.

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產品名稱：重組恒河猴血清淀粉樣蛋白A1蛋白