產品詳情
簡單介紹：
重組鼠CXCL16蛋白與其它公司提供的重組蛋白不同，rMuCXCL16蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠CXCL16蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠CXCL16蛋白
| Synonyms | SR-PXOX, Scavenger Peceptor for Phosphatidylserine and Oxidized Low Density Lipoprotein, Small-inducible Cytokine B16, Transmembrane Chemokine CXCL16 |
| Species | Murine |
| Accession | Q8BSU2 |
| GeneID | 66102 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠CXCL16蛋白Approximately 9.9 kDa, a single non-glycosylated polypeptide chain containing 88 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠CXCL16蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 20-1000 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
| Endotoxin | Less than 1 EU/μg of rMuCXCL16 as determined by LAL method. |
| Reconstitution | 重組鼠CXCL16蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠CXCL16蛋白 | |
| Reference |
1. Wuttge DM, Zhou X, Sheikine Y, et al. 2004. Arterioscler Thromb Vasc Biol, 24: 750-5. 2. Hojo S, Koizumi K, Tsuneyama K, et al. 2007. Cancer Res, 67: 4725-31. 3. Lu Y, Wang J, Xu Y, et al. 2008. Mol Cancer Res, 6: 546-54. 4. Wente MN, Gaida MM, Mayer C, et al. 2008. Int J Oncol, 33: 297-308. 5. Manabe S, Iwase A, Goto M, et al. 2011. Arch Gynecol Obstet, 284: 1567-72. |
| Background | CXCL16 is a member of the CXC chemokine family. Larger than other chemokines, it is one of the only two transmembrane chemokines in the family and the other is CX3CL1. Mouse CXCL16 has 246 a.a. and consists of a 26 a.a. residue putative signal peptide, an 88 a.a. residue chemokine domain, an 87 a.a. residue mucin-like spacer region, a 22 a.a. residue transmembrane domain, and a 23 a.a. residue cytoplasmic tail. Mouse CXCL16 shares 70 % sequence identity with human CXCL16 in chemokine domain. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. Functions of CXCL16 include inducing a strong chemotactic response and calcium mobilization. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. |
