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產品名稱：重組鼠白細胞介素-33蛋白
產品型號：rMuIL-33
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組鼠白細胞介素-33蛋白與其它公司提供的重組蛋白不同，rMuIL-33蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠白細胞介素-33蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠白細胞介素-33蛋白

Synonyms IL-1F11, NF-HEV

Species Murine

Accession Q8BVZ5

GeneID 77125

Source Escherichia coli.

Molecular Weight 重組鼠白細胞介素-33蛋白Approximately 17.5 kDa protein containing 158 amino acid residues.

Quantity 2μg/10μg/1000μg

AA Sequence SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠白細胞介素-33蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, and 1 mM EDTA.

Endotoxin Less than 1 EU/μg of rMuIL-33 as determined by LAL method.

Reconstitution 重組鼠白細胞介素-33蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠白細胞介素-33蛋白

SDS-PAGE

Reference 1. Arend WP, Palmer G, Gabay C. 2008. Immunol Rev, 223: 20-38.
2. Ajdukovic J, Tonkic A, Salamunic I, et al. 2010. Hepatogastroenterology, 57: 1442-4.
3. Ho LH, Ohno T, Oboki K, et al. 2007. J Leukoc Biol, 82: 1481-90.
4. Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46.
5. Blom L, Poulsen BC, Jensen BM, et al. 2011. PLoS One, 6: e21695.

Background IL-33 is a number of IL-1 superfamily, secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Mature IL-33 share approximately 55 % and 90 % a.a. sequence identity with human and rat IL-33 respectively.

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Synonyms	IL-1F11, NF-HEV
Species	Murine
Accession	Q8BVZ5
GeneID	77125
Source	Escherichia coli.
Molecular Weight	重組鼠白細胞介素-33蛋白Approximately 17.5 kDa protein containing 158 amino acid residues.
Quantity	2μg/10μg/1000μg
AA Sequence	SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠白細胞介素-33蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, and 1 mM EDTA.
Endotoxin	Less than 1 EU/μg of rMuIL-33 as determined by LAL method.
Reconstitution	重組鼠白細胞介素-33蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠白細胞介素-33蛋白
SDS-PAGE
Reference	1. Arend WP, Palmer G, Gabay C. 2008. Immunol Rev, 223: 20-38. 2. Ajdukovic J, Tonkic A, Salamunic I, et al. 2010. Hepatogastroenterology, 57: 1442-4. 3. Ho LH, Ohno T, Oboki K, et al. 2007. J Leukoc Biol, 82: 1481-90. 4. Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46. 5. Blom L, Poulsen BC, Jensen BM, et al. 2011. PLoS One, 6: e21695.
Background	IL-33 is a number of IL-1 superfamily, secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Mature IL-33 share approximately 55 % and 90 % a.a. sequence identity with human and rat IL-33 respectively.

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產品名稱：重組鼠白細胞介素-33蛋白