產品詳情
簡單介紹：
重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白與其它公司提供的重組蛋白不同，rHuSDF-1β/CXCL12β蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白
| Synonyms | hSDF-1 beta, IRH, hIRH, PBSF |
| Species | Human |
| Accession | P48061 |
| GeneID | 6387 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | 重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml. |
| Physical Appearance | 重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuSDF-1β/CXCL12β as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類基質細胞衍生因子-1 beta / CXCL12 beta蛋白 | |
| SDS-PAGE |
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| Reference |
1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. Yu L, Cecil J, Peng SB, et al. 2006. Gene. 374:174-9. 3. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 4. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 5. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 6. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 7. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 8. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53. 9. Kryczek I, Wei S, Keller E, et al. 2007. Am J Physiol Cell Physiol. 292:C987-95. |
| Background | CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. In all SDF-1 isoforms, SDF-1β is the canonical sequence. It has the complete amino acids in the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. |
