產品詳情
簡單介紹：
重組人神經營養(yǎng)蛋白-4蛋白與其它公司提供的重組蛋白不同，rHuNT-4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人神經營養(yǎng)蛋白-4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人神經營養(yǎng)蛋白-4蛋白
| Synonyms | Neurotrophin-5, NT-5 |
| Species | Human |
| Accession | P34130 |
| GeneID | 4909 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人神經營養(yǎng)蛋白-4蛋白Approximately 28.1 kDa, a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers (262 total amino acid residues). |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人神經營養(yǎng)蛋白-4蛋白Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5. |
| Endotoxin | Less than 1 EU/μg of rHuNT-4 as determined by LAL method. |
| Reconstitution | 重組人神經營養(yǎng)蛋白-4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人神經營養(yǎng)蛋白-4蛋白 | |
| SDS-PAGE |
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| Reference |
1. Gao WQ, Zheng JL, Karihaloo M. 1995. J Neurosci, 15: 2656-67. 2. Ogborn DIandGardiner PF. 2010. Muscle Nerve, 41: 385-91. 3. Peinado-Ramon P, Salvador M, Villegas-Perez MP, et al. 1996. Invest Ophthalmol Vis Sci, 37: 489-500. 4. Yuen ECandMobley WC. 1999. Exp Neurol, 159: 297-308. 5. Sakuma K, Watanabe K, Sano M, et al. 2001. Brain Res, 907: 1-19. |
| Background | NT-4 also named as NT-5 is a neuronal and epithelial grow factor belongs to the NGF-beta family. The NT-4 precursor is consisted of a 24 a.a. signal peptide, a 56 a.a. propertied and 130 a.a. NT-4. The mature protein has six Cys amino acid residues and has the relative structure with NT-3, BDNF (sharing about 48 % - 52 % sequence identity). Additionally, it shares 91 % and 95 % a.a. sequence identity with mouse and rat NT-4. NT-4 is mainly expressed in prostate and has low level thymus, placenta, and skeletal muscle. It can binding with the LNGFR and trkB receptors and plays a crucial role in the regulation of survival and the maintenance of peripheral sensory sympathetic neurons. Defect of NT-4 may cause primary open angle glaucoma type 1O. |
