產品詳情
簡單介紹：
重組人白細胞介素-1受體拮抗劑蛋白蛋白與其它公司提供的重組蛋白不同，rHuIL-1RA蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-1受體拮抗劑蛋白蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人白細胞介素-1受體拮抗劑蛋白蛋白
| Synonyms | IL-1RN, IRAP |
| Species | Human |
| Accession | P18510 |
| GeneID | 3557 |
| Source | 重組人白細胞介素-1受體拮抗劑蛋白蛋白Escherichia coli. |
| Molecular Weight | Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人白細胞介素-1受體拮抗劑蛋白蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuIL-1RA as determined by LAL method. |
| Reconstitution | 重組人白細胞介素-1受體拮抗劑蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人白細胞介素-1受體拮抗劑蛋白蛋白 | |
| SDS-PAGE |
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| Reference |
1. Dinarello, C.A. 1994. FASEB J, 8: 1314-25. 2. Perrier, S., F. Darakhshan, and E. Hajduch. 2006. FEBS Lett, 580: 6289-94. 3. Dinarello, C.A. 1994. FASEB J, 8: 1314-25. |
| Background | Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family. IL1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-RA. The regulated expression of IL1RA in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RA is markedly enhanced by IL-1, TNF-alpha, or PDGF. The protein shows 26 % amino acid homology to IL1β and 19 % homology to IL1α. |
