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產品名稱：重組大鼠白細胞介素-1 beta蛋白
產品型號：rRtIL-1β
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠白細胞介素-1 beta蛋白與其它公司提供的重組蛋白不同，rRtIL-1β蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細胞介素-1 beta蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠白細胞介素-1 beta蛋白

Synonyms Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP

Species Rat

Accession Q63264

Unigene Rn.9869.

Source Escherichia coli.

Molecular Weight 重組大鼠白細胞介素-1 beta蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠白細胞介素-1 beta蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.

Endotoxin Less than 1 EU/μg of rRtIL-1β as determined by LAL method.

Reconstitution 重組大鼠白細胞介素-1 beta蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠白細胞介素-1 beta蛋白

SDS-PAGE

Reference 1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.
2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.
3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.
4. Gray, P.W., D. Glaister, E. Chen, et al. 1986. J Immunol, 137: 3644-8.

Background Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1αand IL-1β binds to the same receptor and has similar but not identical biological properties. The mature rat IL1β shares 90 % a.a. sequence identity with cotton rat and mouse and 65 % to 77 % with canine, human,and rhesus IL1β.

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Synonyms	Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP
Species	Rat
Accession	Q63264
Unigene	Rn.9869.
Source	Escherichia coli.
Molecular Weight	重組大鼠白細胞介素-1 beta蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠白細胞介素-1 beta蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
Endotoxin	Less than 1 EU/μg of rRtIL-1β as determined by LAL method.
Reconstitution	重組大鼠白細胞介素-1 beta蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠白細胞介素-1 beta蛋白
SDS-PAGE
Reference	1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83. 2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93. 3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22. 4. Gray, P.W., D. Glaister, E. Chen, et al. 1986. J Immunol, 137: 3644-8.
Background	Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1αand IL-1β binds to the same receptor and has similar but not identical biological properties. The mature rat IL1β shares 90 % a.a. sequence identity with cotton rat and mouse and 65 % to 77 % with canine, human,and rhesus IL1β.

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產品名稱：重組大鼠白細胞介素-1 beta蛋白