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產(chǎn)品名稱：重組大鼠心肌營養(yǎng)素-1蛋白
產(chǎn)品型號：rRtCT-1
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組大鼠心肌營養(yǎng)素-1蛋白與其它公司提供的重組蛋白不同，rRtCT-1蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠心肌營養(yǎng)素-1蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠心肌營養(yǎng)素-1蛋白

Species Rat

Accession Q63086

GeneID 29201

Source Escherichia coli.

Molecular Weight 重組大鼠心肌營養(yǎng)素-1蛋白Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組大鼠心肌營養(yǎng)素-1蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.

Endotoxin Less than 1 EU/μg of rRtCT-1 as determined by LAL method.

Reconstitution 重組大鼠心肌營養(yǎng)素-1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠心肌營養(yǎng)素-1蛋白

SDS-PAGE

Reference 1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91.
2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63.
3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.

Background Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

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Species	Rat
Accession	Q63086
GeneID	29201
Source	Escherichia coli.
Molecular Weight	重組大鼠心肌營養(yǎng)素-1蛋白Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組大鼠心肌營養(yǎng)素-1蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.
Endotoxin	Less than 1 EU/μg of rRtCT-1 as determined by LAL method.
Reconstitution	重組大鼠心肌營養(yǎng)素-1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠心肌營養(yǎng)素-1蛋白
SDS-PAGE
Reference	1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91. 2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63. 3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.
Background	Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

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產(chǎn)品名稱：重組大鼠心肌營養(yǎng)素-1蛋白